An endophytic fungus lives in mycelial form in biological association with the living plant, at least for some time. Some species of endophytic fungi have been identified as sources of anticancer, antidiabetic, insecticidal and immunosuppressive compounds. Here we investigated endophytic fungi associated with the plants Nerium oleander L. and Thevetia peruviana (Apocynaceae). Total 22 endophytic fungi were isolated from these plants. The antidiabetic activity was determined for two reprehensive species grown on improved Czapek’s broth medium. These endophytic fungi showed a mild antidiabetic activity. Our study showed that the endophytic fungi from Nerium oleander L. and Thevetia peruviana are potential source of the novel antidiabetic compounds.
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